Abstract Detail

Recent Topics Posters

Creer , Marylyn Height [1], Holappa, Lynn [2], Kramer, Elena M [3].

Studying Protein Interactions Among MADS-box Transcription Factors.

THE MADS-BOX DOMAIN transcription factors are key to determining the development of plants and are not only important for plant evolution and organ development, but are paramount in studying determining factors in the development of most eukaryotes.  In studying Arabidopsis, the ABC model for plant development has been described, with the A, B and C gene classes specifying the major floral organs.  In studying flower development,  it is very important to analyze factors that contribute to overall plant evolution and organ development.  The species Aquilegia (columbine) is being used as a model to study how MADS box genes have contributed to the evolution of the ABC model. This works starts with the use of miniprep assays to isolate plasmid DNA from E. coli cultures.  Plasmid DNA is then used in enzyme digestion, cloning, PCR, transcription, and conventional and automated sequencing.  This particular process is used to obtain DNA constructs that can then be used to perform continuing analyses, including Yeast Two Hybrid (Y2H) analysis, as well as the use of GFP (Green Fluorescent Protein) in order to assess protein interactions in yeast and in vivo. The broad goal of this project is to determine as accurately as possible the genetic basis of flower organ development in Aquilegia and its evolutionary significance.  

1 - Alabama A&M University, Biological and Environmental Life Sciences, P.O. Box 132, Normal, Alabama, 37562, United States
2 - Harvard University, Organismic and Evolutionary Biologyl, 16 Divinity Av, Cambridge, Ma, 02138, USA
3 - Harvard University, 16 Divinity Avenue, Cambridge, MA, 02138, USA

Keywords:
MADS-box
Transcription 
Factors.

Presentation Type: Recent Topics Poster
Session: P
Location: /
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PRT014
Abstract ID:1251
Candidate for Awards:None