Abstract Detail

Genomics / Proteomics

Nepal, Madhav P. [1], Benson, Benjamin V. [2], Davidson , Lukas G.  [2], Moore, Brian G. [3].

Evolution of  R-gene Core Promoter Elements in Plants.

Plant proteins encoded by disease resistance genes (R-genes) are involved in detecting pathogen-attack and activating downstream defense molecules. The main objectives of this study were to identify promoters 2kbp upstream of CNL (Coiled-Coil Nucleotide binding site Leucine rich region) type of R-genes and elucidate their distribution and diversity across six plant species. We searched 913 CNL genes across the plant genomes—Rice, Soybean, Poplar, Alfalfa, Common Bean, and Arabidopsis using 469 common promoters available at the PLACE database. Using in-house Perl scripts the sequence data were parsed yielding the following: 327 of 469 promoter regions were found in the described region; eight of our promoters were common to all genes including the most abundant promoter DOFCOREZM appearing 27619 times; and 13 promoters had a frequency per gene of greater than 10. Only monocot species included in this study had lower number of promoters than dicots species. Plant genomes are generally known to lack a universal promoter, but the CNL R-genes in the present study had eight promoter regions that appeared in the 2kbp up-stream region of each of the 913 genes sampled. Since the core promoters are involved in initiating transcription process, their identification would have future implication in developing durable resistance genes useful in crop improvement.

Related Links:
Nepal Lab at SDSU

1 - South Dakota State University, Biology And Microbiology, Northern Plain Biostress Laboratory, Brookings, SD, 57007, USA
2 - South Dakota State University, Biology and Microbiology, Brookings, SD, 57007, USA
3 - South Dakota State University, Univ Networking Systems & Services, Brookings, SD, 57007

Keywords:
Disease Resistance Genes or R-genes
Core Promotor Elements
CNL Genes
Evolutionary Anaysis.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Eyrie/Boise Centre
Date: Monday, July 28th, 2014
Time: 5:30 PM
Number: PGP001
Abstract ID:289
Candidate for Awards:Genetics Section Poster Award